High frequency perfusion fluorometry (HFPF) to assess bronchial mucosa blood perfusion

120.High frequency perfusion fluorometry (HFPF) preliminary results on a new technique to assess bronchial mucosa blood perfusion.

G.RESCIGNO, S. NAZARI, L. BARANA, U. PRATI, G. BOTTIROLI, A. ZONTA

Abstract, Proceedings of the 5th Annual Meeting of The European Association for CardioThoracic Surgery. London Sept 22-25, 1991

120-I.High frequency perfusion fluorometry (HFPF) preliminary results on a new technique to assess bronchial mucosa blood perfusion.

G.RESCIGNO,G. BOTTIROLI S. NAZARI, C. GEROSA, A.RINALDO, R.RAMPONI, A.BERTI, AC CROCE, A. ZONTA

Abstract, Proceedings 26th Congress and 8th Tripartite Meeting European Society for Surgical Research, Salzburg, May 8-11, 1991

EUR SURG RES 23 (S1) : 95-96WXS, 1991 I.F. 0,782

120-II-.High frequency perfusion fluorometry (HFPF) preliminary results on a new technique to assess bronchial mucosa blood perfusion.

G.RESCIGNO, S. NAZARI, L. BARANA, G. BOTTIROLI, P.FILISETTI, F TANZI, A. ZONTA

Abstract, Proceedings of the 2nd International Lung Transplant Seminar, Zurich, June 27/28, 1991

126.High frequency perfusion fluorometry (HFPF) preliminary results on a new technique to assess bronchial mucosa blood perfusion.

G. BOTTIROLI, R. RAMPONI, AC. CROCE, G. RESCIGNO, S.NAZARI, A. ZONTA

Proceedings of SPIE , Los Angeles, 22-23 Jan 1992,Volume 1641:238-245, 1992

145. High frequency perfusion fluorometry (HFPF) preliminary results on a new technique to assess bronchial mucosa blood perfusion.

G. RESCIGNO, S. NAZARI, A. BERTI, A. ZONTA and G. BOTTIROLI

J. SURG RES, 55: 524-530, 1993 I.F. 1,156

A new method for estimating airway mucosa bloodperfusion is proposed. It is based on the use of a high-sensitivity perfusion fluorometer, which allows us todetect the first intravascular passage of injected fluoro-chromes. Total measuring time can be sensibly reducedwith respect to standard perfusion fluorometry, allow-ing us to make assessments during fiberoptic bronchos-copy. In vitro studies were performed to set up the opti-cal arrangement of the instrument. The optimal mea-suring distance (2.2 mm between the probe tip and thefluorescent mucosa was established using a fluorescentfilm surface to simulate fluorescent mucosa. The depthof tissue layers involved in the fluorescence emissionwas assessed by positioning tissue sections of differentthickness between the probe tip and the fluorescentfilm. Tissue layers at depths not exceeding 1200 ,umwere found to contribute to the fluorescence signal re-corded by the instrument. Mucosa blood perfusion wasevaluated in rat trachea, as a model, under three differ-ent experimental conditions of vascularization: (a)basal condition (BC), (b) partial devascularization(PD), (c) full devascularization (FD). Sodium fluores-cein (diffusible into the interstice) and fluoresceinisothiocyanate?dextran (not diffusible into the inter-stice) were used as fluorescent tracers. Under the BCboth substances allowed us to detect the first passage offluorochrome through the mucosa capillary networkwith the same accuracy. In PD the first pass peak dis-appeared; total emitted fluorescence decreased as well.In FD a flat tracing was recorded. Consistent resultswere obtained with both substances. Our resultsshowed that high-frequency perfusion fluorometry canbe used as an alternative method for assessing bron-chial mucosa blood perfusion after lung transplantation.

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